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KMID : 0545119990090030361
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 3 p.361 ~ p.364
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Biochemical Analysis of Anagrapha falcifera NPV Attachment to Spodoptera frugiperda 21 Cells
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Park, Jin O
Yang, Jai Myung/Chung, In Sik
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Abstract
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The binding characteristics of Anagrapha falcifera nuclear polyhedrosis virus (AfNPV) to Spodoptera frugiperda 21 (Sf21) cells were investigated. The cells displayed an affinity of 4.7×10 exp(10) M^-1 with about 3,300 binding sites per cell. The biochemical nature of the AfNPV-binding sites on the cell surface was also partially identified. Our findings suggest that the binding-site moiety has a glycoprotein component, but that the direct involvement of oligosacccharides containing N-acetylglucosamine or sialic acid residues in binding is unlikely, and that AfNPV entry into Sf2l cells may be via receptor-mediated endocytosis.
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